TAE (Tris-Acetate-EDTA , 20X, 50X) Buffer is the most commonly used buffer for agarose DNA electrophoresis, and originally used at a working strength of 1X or 0.5X for agarose gel electrophoresis.
Premixed electrophoresis buffers eliminate variables which can distort data and give unreliable results.Premixed buffers are made from the high quality reagents and are carefully mixed and tested to ensure reproducibility.
Composition(20X)- 800 mM Tris-acetate- 20 mM EDTA- pH at 25°C (1X) : 8.2-8.4.(50X)- 2 M Tris-acetate- 50 mM EDTA- pH at 25°C (1X) : 8.2-8.4. Recommended Storage Condition- Store at Temperature (22–25°C) Usage Information (applications)
- Electrophoresis buffer for agarose gel electrophoresis