6x DNA Loading Buffer is a pre-mixed loading buffer with two tracking dyes for agarose and non-denaturing poylacrylamide gel electrophoresis.
The dye is used for loading DNA samples into gel electrophoresis wells and tracking migration during electrophoresis.In a 0.5–1.4% agarose gel in 0.5x TBE, xylene cyanol FF migrates at approximately 4 kb and bromophenol blue at approximately 300 bp.
6x Composition- 30% Glycerol, 0.25% Xylene cyanol FF, 0.25% Bromophenol Blue.Recommended Storage Condition- Store at room temperature (22–25°C)Usage Information- Use 1 ㎕ DNA Loading Buffer (6x) per 5 ㎕ DNA solution. Mix well before loading gel.