ELISA titer

 

     

 

Titration ELISA demonstratingspecificbinding of Anti-Nectin4-rIgG #42 (sdAb) to Nectin4.

Nectin4 coatedat 2 μg/ml; antibodyseriallydilutedfrom 1 μg/ml; detectedbyindirect ELISA (OD₄₅₀).

Noreactivityagainst BSA control (○).

 

EC₅₀ = 0.0087 μg/ml (●).

 

Antibody Format : Camelid VHH(sdAb) #42

 

Fusion Protein : rabbit-IgG

 

Antigen : Nectin-4

 

UniProtKB : Q96NY8

 

Species : Human

 

Species Cross-reactivity :

 

Remarks :

 

Applications : Dilution

 

  ● ELISA

 

  ● WB  _

 

  ● IF  _

 

Description :

 

  ● Antigen Preparation : 

 

  ● Antigen Amino Acid Sequence : 

 

Abbrevications and cautions :

  

  ● scFv : human single chain antibody (naive sequences) 

 

  ● sdAb : camel single domain antibody (fully synthetic sequences) 

 

  ● sVH : synthetic human variable heavy domain   

 

  ● GB1 fusion : IgG binding domain of streptococcal protein G 

 

  ● IgG Fc fusion : human, mouse or rabbit IgG Fc (contains hinge, CH1, CH2 domain) 

 

  ● Applications : Applications : yes/no or _ (not determined)