Antibody Format : sdAb

 

Fusion Protein : GB1

 

Antigen : CRISPR-associated endonuclease Cas9/Csn1

 

UniProtKB : Q99ZW2

 

Species : STRP1

 

Species Cross-reactivity :​

 

Remarks :​

 

Applications : Dilution

 

  ● ELISA

 

  ● ​WB  1/1,000

 

  ● ​IF  _

​

Description :

 

  ● ​Antigen Preparation : The 158.3 kDa full length protein was expressed in E.coli and purified by his affinity purification.

 

  ● ​Antigen Amino Acid Sequence : D K K Y S I G L D I G T N S V G W A V I T D E Y K V P S K K F K V L G N T D R H S I K K N L I G A L L F D S G E T A E A T R L K R T A R R R Y T R R K N R I C Y L Q E I F S N E M A K V D D S F F H R L E E S F L V E E D K K H E R H P I F G N I V D E V A Y H E K Y P T I Y H L R K K L V D S T D K A D L R L I Y L A L A H M I K F R G H F L I E G D L N P D N S D V D K L F I Q L V Q T Y N Q L F E E N P I N A S G V D A K A I L S A R L S K S R R L E N L I A Q L P G E K K N G L F G N L I A L S L G L T P N F K S N F D L A E D A K L Q L S K D T Y D D D L D N L L A Q I G D Q Y A D L F L A A K N L S D A I L L S D I L R V N T E I T K A P L S A S M I K R Y D E H H Q D L T L L K A L V R Q Q L P E K Y K E I F F D Q S K N G Y A G Y I D G G A S Q E E F Y K F I K P I L E K M D G T E E L L V K L N R E D L L R K Q R T F D N G S I P H Q I H L G E L H A I L R R Q E D F Y P F L K D N R E K I E K I L T F R I P Y Y V G P L A R G N S R F A W M T R K S E E T I T P W N F E E V V D K G A S A Q S F I E R M T N F D K N L P N E K V L P K H S L L Y E Y F T V Y N E L T K V K Y V T E G M R K P A F L S G E Q K K A I V D L L F K T N R K V T V K Q L K E D Y F K K I E C F D S V E I S G V E D R F N A S L G T Y H D L L K I I K D K D F L D N E E N E D I L E D I V L T L T L F E D R E M I E E R L K T Y A H L F D D K V M K Q L K R R R Y T G W G R L S R K L I N G I R D K Q S G K T I L D F L K S D G F A N R N F M Q L I H D D S L T F K E D I Q K A Q V S G Q G D S L H E H I A N L A G S P A I K K G I L Q T V K V V D E L V K V M G R H K P E N I V I E M A R E N Q T T Q K G Q K N S R E R M K R I E E G I K E L G S Q I L K E H P V E N T Q L Q N E K L Y L Y Y L Q N G R D M Y V D Q E L D I N R L S D Y D V D H I V P Q S F L K D D S I D N K V L T R S D K N R G K S D N V P S E E V V K K M K N Y W R Q L L N A K L I T Q R K F D N L T K A E R G G L S E L D K A G F I K R Q L V E T R Q I T K H V A Q I L D S R M N T K Y D E N D K L I R E V K V I T L K S K L V S D F R K D F Q F Y K V R E I N N Y H H A H D A Y L N A V V G T A L I K K Y P K L E S E F V Y G D Y K V Y D V R K M I A K S E Q E I G K A T A K Y F F Y S N I M N F F K T E I T L A N G E I R K R P L I E T N G E T G E I V W D K G R D F A T V R K V L S M P Q V N I V K K T E V Q T G G F S K E S I L P K R N S D K L I A R K K D W D P K K Y G G F D S P T V A Y S V L V V A K V E K G K S K K L K S V K E L L G I T I M E R S S F E K N P I D F L E A K G Y K E V K K D L I I K L P K Y S L F E L E N G R K R M L A S A G E L Q K G N E L A L P S K Y V N F L Y L A S H Y E K L K G S P E D N E Q K Q L F V E Q H K H Y L D E I I E Q I S E F S K R V I L A D A N L D K V L S A Y N K H R D K P I R E Q A E N I I H L F T L T N L G A P A A F K Y F D T T I D R K R Y T S T K E V L D A T L I H Q S I T G L Y E T R I D L S Q L G G D

 

​Abbrevications and cautions :

 

  ● ​scFv : human single chain antibody (naive sequences)

 

  ● ​sdAb : camel single domain antibody (fully synthetic sequences)

 

  ● ​GB1 fusion : IgG binding domain of streptococcal protein G

 

  ● ​IgG Fc fusion : human, mouse or rabbit IgG Fc (contains hinge, CH1, CH2 domain)

 

  ● ​Applications : yes/no or _ (not determined)