Antibody Format : sdAb
Fusion Protein : GB1
Antigen : Chaperone protein HscA
UniProtKB : P0A6Z1
Species : E.coli
Species Cross-reactivity :
Remarks :
Applications : Dilution
● ELISA
● WB 1/1,000
● IF _
Description :
● Antigen Preparation : The 66 kDa full length protein was expressed in E.coli and purified by his affinity purification.
● Antigen Amino Acid Sequence :
M A L L Q I S E P G L S A A P H Q R R L A A G I D L G T T N S L V A T V R S G Q A E T L A D H E G R H L L P S V V H Y Q Q Q G H S V G Y D A R T N A A L D T A N T I S S V K R L M G R S L A D I Q Q R Y P H L P Y Q F Q A S E N G L P M I E T A A G L L N P V R V S A D I L K A L A A R A T E A L A G E L D G V V I T V P A Y F D D A Q R Q G T K D A A R L A G L H V L R L L N E P T A A A I A Y G L D S G Q E G V I A V Y D L G G G T F D I S I L R L S R G V F E V L A T G G D S A L G G D D F D H L L A D Y I R E Q A G I P D R S D N R V Q R E L L D A A I A A K I A L S D A D S V T V N V A G W Q G E I S R E Q F N E L I A P L V K R T L L A C R R A L K D A G V E A D E V L E V V M V G G S T R V P L V R E R V G E F F G R P P L T S I D P D K V V A I G A A I Q A D I L V G N K P D S E M L L L D V I P L S L G L E T M G G L V E K V I P R N T T I P V A R A Q D F T T F K D G Q T A M S I H V M Q G E R E L V Q D C R S L A R F A C V V F R R Y R L A G A H I R V T F Q V D A D G L L S V T A M E K S T G V E A S I Q V K P S Y G L T D S E I A S M I K D T M S Y A E Q D V K A R M L A E Q K V E A A R V L E S L H G A L A A D A A L L S A E R Q V I D D A A A H L S E V A Q G D D V D A I E Q A I K N V D K Q T Q D F A A R R M D Q S V R R A L K G H S V D E V
Abbrevications and cautions :
● scFv : human single chain antibody (naive sequences)
● sdAb : camel single domain antibody (fully synthetic sequences)
● GB1 fusion : IgG binding domain of streptococcal protein G
● IgG Fc fusion : human, mouse or rabbit IgG Fc (contains hinge, CH1, CH2 domain)
● Applications : yes/no or _ (not determined)
