Antibody Format : sdAb
Fusion Protein : GB1
Antigen : E.coli ATP-dependent DNA helicase RecQ
UniProtKB : P15043
Species : E.coli
Species Cross-reactivity :
Remarks :
Applications : Dilution
● ELISA
● WB _
● IF _
Description :
● Antigen Preparation : The 68.3 kDa full length protein was expressed in E.coli and purified by his affinity purification.
● Antigen Amino Acid Sequence :
AQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCLVVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTREQQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRPEYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLMEKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSKGISAAAYHAGLENNVRADVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPAEAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEGRQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDYGHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSLQLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEMAEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE
Abbrevications and cautions :
● scFv : human single chain antibody (naive sequences)
● sdAb : camel single domain antibody (fully synthetic sequences)
● GB1 fusion : IgG binding domain of streptococcal protein G
● IgG Fc fusion : human, mouse or rabbit IgG Fc (contains hinge, CH1, CH2 domain)
● Applications : yes/no or _ (not determined)