Antibody Format : sdAb

Fusion Protein : GB1

Antigen : NusA

UniProtKB : P0AFF6

Species : E.coli

Species Cross-reactivity : 

Remarks :

Applications : Dilution

○ ELISA

○ WB 1/1,000

○ IF _

 

Description

Antigen Preparation : The 54.9 kDa full length protein from pDEST-NusA was expressed in E.coli and purified by his affinity purification.

Antigen Amino Acid Sequence : 

M N K E I L A V V E A V S N E K A L P R E K I F E A L E S A L A T A T K K K Y E Q E I D V R V Q I D R K S G D F D T F R R W L V V D E V T Q P T K E I T L E A A R Y E D E S L N L G D Y V E D Q I E S V T F D R I T T Q T A K Q V I V Q K V R E A E R A M V V D Q F R E H E G E I I T G V V K K V N R D N I S L D L G N N A E A V I L R E D M L P R E N F R P G D R V R G V L Y S V R P E A R G A Q L F V T R S K P E M L I E L F R I E V P E I G E E V I E I K A A A R D P G S R A K I A V K T N D K R I D P V G A C V G M R G A R V Q A V S T E L G G E R I D I V L W D D N P A Q F V I N A M A P A D V A S I V V D E D K H T M D I A V E A G N L A Q A I G R N G Q N V R L A S Q L S G W E L N V M T V D D L Q A K H Q A E A H A A I D T F T K Y L D I D E D F A T V L V E E G F S T L E E L A Y V P M K E L L E I E G L D E P T V E A L R E R A K N A L A T I A Q A Q E E S L G D N K P A D D L L N L E G V D R D L A F K L A A R G V C T L E D L A E Q G I D D L A D I E G L T D E K A G A L I M A A R N I C W F G D E A

 

Abbrevications and cautions

○ scFv : human single chain antibody (naive sequences)

○ sdAb : camel single domain antibody (fully synthetic sequences)

○ GB1 fusion : IgG binding domain of streptococcal protein G

○ IgG Fc fusion : human, mouse or rabbit IgG Fc (contains hinge, CH1, CH2 domain)

○ Applications : yes/no or _ (not determined)​