Antibody Format : sdAb
Fusion Protein : GB1
Antigen : Protein disulfide-isomerase
UniProtKB : P07237
Species : HUMAN
Species Cross-reactivity :
Remarks :
Applications : Dilution
○ ELISA
○ WB 1/1,000
○ IF -
Description
Antigen Preparation : The 27.5 kDa protein domain (237-475 aa) was expressed in E.coli and purified by his affinity purification.
Antigen Amino Acid Sequence :
V I E F T E Q T A P K I F G G E I K T H I L L F L P K S V S D Y D G K L S N F K T A A E S F K G K I L F I F I D S D H T D N Q R I L E F F G L K K E E C P A V R L I T L E E EMT K Y K P E S E E L T A E R I T E F C H R F L E G K I K P H LMS Q E L P E D W D K Q P V K V L V G K N F E D V A F D E K K N V F V E F Y A P W C G H C K Q L A P I W D K L G E T Y K D H E N I V I A KMD S T A N E V E A V K V H S F P T L K F F P A S A D R T V I D Y N G E R T L D G F K K F L E S G G Q
Abbrevications and cautions
○ scFv : human single chain antibody (naive sequences)
○ sdAb : camel single domain antibody (fully synthetic sequences)
○ GB1 fusion : IgG binding domain of streptococcal protein G
○ IgG Fc fusion : human, mouse or rabbit IgG Fc (contains hinge, CH1, CH2 domain)
○ Applications : yes/no or _ (not determined)