Antibody Format : sdAb
Fusion Protein : GB1
Antigen : Protein disulfide-isomerase
UniProtKB : P07237
Species : HUMAN
Species Cross-reactivity :
Remarks :
Applications : Dilution
○ ELISA
○ WB 1/1,000
○ IF -
Description
Antigen Preparation : The 23.8 kDa protein domain (18-236 aa) was expressed in E.coli and purified by his affinity purification.
Antigen Amino Acid Sequence :
A P E E E D H V L V L R K S N F A E A L A A H K Y L L V E F Y A P W C G H C K A L A P E Y A K A A G K L K A E G S E I R L A K V D A T E E S D L A Q Q Y G V R G Y P T I K F F R N G D T A S P K E Y T A G R E A D D I V N W L K K R T G P A A T T L P D G A A A E S L V E S S E V A V I G F F K D V E S D S A K Q F L Q A A E A I D D I P F G I T S N S D V F S K Y Q L D K D G V V L F K K F D E G R N N F E G E V T K E N L L D F I K H N Q L
Abbrevications and cautions
○ scFv : human single chain antibody (naive sequences)
○ sdAb : camel single domain antibody (fully synthetic sequences)
○ GB1 fusion : IgG binding domain of streptococcal protein G
○ IgG Fc fusion : human, mouse or rabbit IgG Fc (contains hinge, CH1, CH2 domain)
○ Applications : yes/no or _ (not determined)