Antibody Format : sdAb

Fusion Protein : GB1

Antigen : Lamin-A/C

UniProtKB : P02545

Species : HUMAN

Species Cross-reactivity : human, mouse

Remarks : Cross-react against Laminin B1 and B2

Applications : Dilution

○ ELISA

○ WB 1/1,000

○ IF 1/200

 

Description

Antigen Preparation : The 65.1 kDa human full length protein was expressed in E.coli and purified by his affinity purification.

Antigen Amino Acid Sequence : 

M E T P S Q R R A T R S G A Q A S S T P L S P T R I T R L Q E K E D L Q E L N D R L A V Y I D R V R S L E T E N A G L R L R I T E S E E V V S R E V S G I K A A Y E A E L G D A R K T L D S V A K E R A R L Q L E L S K V R E E F K E L K A R N T K K E G D L I A A Q A R L K D L E A L L N S K E A A L S T A L S E K R T L E G E L H D L R G Q V A K L E A A L G E A K K Q L Q D E M L R R V D A E N R L Q T M K E E L D F Q K N I Y S E E L R E T K R R H E T R L V E I D N G K Q R E F E S R L A D A L Q E L R A Q H E D Q V E Q Y K K E L E K T Y S A K L D N A R Q S A E R N S N L V G A A H E E L Q Q S R I R I D S L S A Q L S Q L Q K Q L A A K E A K L R D L E D S L A R E R D T S R R L L A E K E R E M A E M R A R M Q Q Q L D E Y Q E L L D I K L A L D M E I H A Y R K L L E G E E E R L R L S P S P T S Q R S R G R A S S H S S Q T Q G G G S V T K K R K L E S T E S R S S F S Q H A R T S G R V A V E E V D E E G K F V R L R N K S N E D Q S M G N W Q I K R Q N G D D P L L T Y R F P P K F T L K A G Q V V T I W A A G A G A T H S P P T D L V W K A Q N T W G C G N S L R T A L I N S T G E E V A M R K L V R S V T V V E D D E D E D G D D L L H H H H V S G S R R

 

Abbrevications and cautions

○ scFv : human single chain antibody (naive sequences)

○ sdAb : camel single domain antibody (fully synthetic sequences)

○ GB1 fusion : IgG binding domain of streptococcal protein G

○ IgG Fc fusion : human, mouse or rabbit IgG Fc (contains hinge, CH1, CH2 domain)

○ Applications : yes/no or _ (not determined)