Antibody Format : sdAb
Fusion Protein : GB1
Antigen : Vascular endothelial growth factor
UniProtKB : P15692
Species : HUMAN
Species Cross-reactivity :
Remarks :
Applications : Dilution
○ ELISA
○ WB _
○ IF _
Description
Antigen Preparation : The 19.2 kDa human VEGF alpha with 6xhis tag at the N-terminal end was expressed in E.coli and purified by his affinity purification.
Antigen Amino Acid Sequence :
A P M A E G G G Q N H H E V V K F M D V Y Q R S Y C H P I E T L V D I F Q E Y P D E I E Y I F K P S C V P L M R C G G C C N D E G L E C V P T E E S N I T M Q I M R I K P H Q G Q H I G E M S F L Q H N K C E C R P K K D R A R Q E N P C G P C S E R R K H L F V Q D P Q T C K C S C K N T D S R C K A R Q L E L N E R T C R C D K P R R
Abbrevications and cautions
○ scFv : human single chain antibody (naive sequences)
○ sdAb : camel single domain antibody (fully synthetic sequences)
○ GB1 fusion : IgG binding domain of streptococcal protein G
○ IgG Fc fusion : human, mouse or rabbit IgG Fc (contains hinge, CH1, CH2 domain)
○ Applications : yes/no or _ (not determined)