EzPCR™ qPCR 2x Master Mix(SYBR green, Low Rox) is a mixture of thermostable DNA polymerase, dNTPs, SYBR green I dye, reaction buffer, and stabilizer. Users can perform real-time PCR by simply adding template cDNA or gDNA and specific primer pair to the master mix. EzPCR™ qPCR 2x Master Mix(SYBR green, Low Rox) has been optimized for highly sensitive and reproducible real-time PCR experiments. The composition has been optimized to reduce non-specific amplification and to minimize primer-dimer formation.
EzPCR™ qPCR 2x Master Mix(SYBR green, Low Rox) is provided as a 2x concentrated format. Experiment can be simply set by mixing half volume of 2x master mix with primer pair/template DNA solution. 1 ml 2x master mix is sufficient for 100 reactions in a 20 ㎕ standard reaction.
ROX reference dye containing product (EBT-1802) is suitable for a real-time PCR instruments requiring ROX passive reference dye (like company ABI real-time PCR machines). The use of ROX reference dye is optional. If your real-time machine has the capability of using ROX and you wish to use this option, then you can select this option in the software.
ApplicationsReal-Time quantitative RT-PCR and genomic DNA PCR.
Store at -20℃ for one year. After thawing mix, do not freeze again and store at 4℃ in a light protective condition. When stored under optimum conditions, the reagents are stable for a minimum of 6 months from date of purchase.