HS qPCR 2x Master Mix (SYBR green)  includes all kind of real-time RT-qPCR components such as Taq DNA polymerase, dNTP and buffer. The only need to perform the reaction is template cDNA or gDNA and primers provided by users.  

   HS qPCR 2x Master Mix (SYBR) has been optimized for highly sensitive and reproducible real-time PCR experiments. The composition has been optimized to reduce non-specific amplification and to minimize primer-dimer formation. In addition, Taq polymerase is a mixture of PCR enzyme and monoclonal antibody to Taq DNA polymerase. The antibody inhibits the polymerase activity and prevent non-specific amplification derived from mis-priming of primer dimer before PCR cycling.

 

Application(s)

Real-Time quantification of DNA and cDNA targets

Multiplex PCR

Diverse applications using qPCR

Gene expression profiling

 

Characteristics

Enhanced specificity by use of HOT start with anti-Taq antibody.

 

Qualifying Test

Performance test, exo/endo nuclease contamination test, stability test.

 

Storage Condition

For long term storage, we recommend to store at -20℃ (stable up to 12 months), and store at 4℃ after thawing the solution instead of -20 ℃ (Keep the light out).

 

Standard Experiment Protocol