EzAmpTM One-step RT-qPCR 4x Master Mix includes all kind of real-time RT-qPCR components such as Taq DNA polymerase, thermostable M-MLV reverse transcriptase, dNTP and buffer. The only need to perform the reaction is template RNA, probes and primers provided by users. It is only need to perform quantitative RT-qPCR by simply adding total or poly(A) RNA as substrates and specific primer pair to the premix.
Especially, this product is suitable to perform the real-time qPCR reaction, since it has Taq DNA polymerase having the 5’ to 3’ exonuclease activity and designed to detect multiple targets at the same time.
- One-step RT-qPCR
- Multiplex PCR
Performance test, exo/endo nuclease contamination test, stability test.
For long term storage, we recommend to store at -20℃ (stable up to 6 months), and store at 4℃ after thawing the solution instead of -20℃.
Standard Experiment Protocol