HS One-step RT-qPCR 2X Master Mix (SYBR
green, low ROX) includes all kind of
real-time RT-qPCR components such as Taq DNA polymerase, thermostable M-MLV
reverse transcriptase, dNTP,
buffer and SYBR Green.
It is only needed to perform
by simply adding total or poly(A) RNA as substrates and specific primer pair to
HS One-step RT-qPCR 2X Master Mix (SYBR green,
low ROX) has been optimized for highly sensitive and reproducible real-time PCR
In addition, Taq polymerase
PCR enzyme and monoclonal antibody to Taq DNA polymerase. The antibody inhibits
the polymerase activity and prevent non-specific amplification derived from
mis-priming of primer dimer before PCR cycling.
Enhanced specificity by use of HOT start with anti-Taq antibody
50 nM/rxn ROX
One-step RT-qPCR (SYBR)
Performance test, exo/endo nuclease contamination test, stability test.
at -20 ℃ (stable up to 12 months).
repeated freeze-thaw cycle. Keep the
Standard Experiment Protocol