HS One-step RT-qPCR 2X Master Mix (SYBR green, low ROX)  includes all kind of real-time RT-qPCR components such as Taq DNA polymerase, thermostable M-MLV reverse transcriptase, dNTP, buffer and SYBR Green. 

 

It is only needed to perform quantitativeRT-qPCR by simply adding total or poly(A) RNA as substrates and specific primer pair to the premix.

 

HS One-step RT-qPCR 2X Master Mix (SYBR green, low ROX) has been optimized for highly sensitive and reproducible real-time PCR experiments.

 

In addition, Taq polymerase is amixtureof PCR enzyme and monoclonal antibody to Taq DNA polymerase. The antibody inhibits the polymerase activity and prevent non-specific amplification derived from mis-priming of primer dimer before PCR cycling.

 

 

 

 

Characteristics

 - Enhanced specificity by use of HOT start with anti-Taq antibody

 - Includes 50 nM/rxn ROX reference dye

 

 

 

 

 

Application(s)

One-step RT-qPCR (SYBR) 

 

 

 

 

 

Qualifying Test

Performance test, exo/endo nuclease contamination test, stability test.

 

 

 

 

 

Storage Condition

Store at -20 ℃ (stable up to 12 months).

Avoid repeated freeze-thaw cycle.  Keep the light out.

 

 

 

 

 

Standard Experiment Protocol