One-step RT-qPCR 2x Master Mix (SYBR green) includes all kind of real-time RT-qPCR components such as Taq DNA polymerase, thermostable M-MLV reverse transcriptase, dNTP and buffer. The only need to perform the reaction is template RNA and primers provided by users. It is only need to perform quantitative RT-qPCR by simply adding total or poly(A) RNA as substrates and specific primer pair to the premix.
One-step RT-qPCR 2x Master Mix (SYBR) has been optimized for highly sensitive and reproducible real-time PCR experiments. The composition has been optimized to reduce non-specific amplification and to minimize primerdimer formation.
In addition, Taq polymerase is a mixture of PCR enzyme and monoclonal antibody to Taq DNA polymerase. The antibody inhibits the polymerase activity and prevent nonspecific amplification derived from mis-priming of primer dimer before PCR cycling.
- Enhanced specificity by use of HOT start with anti-Taq antibody
- One-step RT-qPCR
- Multiplex PCR
Performance test, exo/endo nuclease contamination test, stability test.
For long term storage, we recommend to store at -20℃ (stable up to 6 months), and store at 4℃ after thawing the solution instead of -20℃.
Standard Experiment Protocol