Description

   One-step RT-qPCR 2x Master Mix (SYBR green) includes all kind of real-time RT-qPCR components such as Taq DNA polymerase, thermostable M-MLV reverse transcriptase, dNTP and buffer. The only need to perform the reaction is template RNA and primers provided by users. It is only need to perform quantitative RT-qPCR by simply adding total or poly(A) RNA as substrates and specific primer pair to the premix.

One-step RT-qPCR 2x Master Mix (SYBR) has been optimized for highly sensitive and reproducible real-time PCR experiments. The composition has been optimized to reduce non-specific amplification and to minimize primerdimer formation.

In addition, Taq polymerase is a mixture of PCR enzyme and monoclonal antibody to Taq DNA polymerase. The antibody inhibits the polymerase activity and prevent nonspecific amplification derived from mis-priming of primer dimer before PCR cycling. 

 

Characteristics

 

- Enhanced specificity by use of HOT start with anti-Taq antibody 

 

Application(s)

 

 

- Multiplex PCR 

 

Qualifying Test

 

Performance test, exo/endo nuclease contamination test, stability test.

 

Storage Condition

For long term storage, we recommend to store at -20℃ (stable up to 6 months), and store at 4℃ after thawing the solution instead of -20℃.

 

Standard Experiment Protocol